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After incubation, the culture medium was collected, centrifuged at 2,000 g for 10 min to remove cellular debris, and the supernatant was carefully harvested for subsequent cytokine quantification using enzyme-linked immunosorbent assays (ELISA) kits: Mouse TNF alpha SimpleStep ELISA Kit (ab208348

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doi: 10.1161/CIRCRESAHA.121.318093 42 KimYWangWOklaMKangIMoreauRChungS

Others who may not be able to tolerate dosages of vitamin B12 that are higher than what is found in most multivitamins include people who have been diagnosed with low potassium, Leber's vision loss, or atrophic gastritis, according to WebMD