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LR3 enables a more reproducible protocol with fewer interventions
Menopause brings about symptoms such as mood changes, fatigue, decreased bone mass, and urinary incontinence
Estimation of liver function parameters Aspartate aminotransferase (AST), alanine aminotransferase (ALT) were assayed in serum samples obtained from all groups of mice by a colorimetric method of Reitman and Frankel (Reitman and Frankel, 1957), while albumin was assessed according to the method of Pinnell and Northam (Pinnell and Northam, 1978) using commercially available kits (Diamond Diagnostic, Egypt) and the Erba CHEM-7 device (ERBA Diagnostics, India)
Two separate intravenous (IV) catheters were inserted, one in each arm, and sometimes an ultrasound was used to locate the venous access in the patients arm
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