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The inserts were removed, the wells were washed with phenol red-free DMEM/HAMs F-12 medium supplemented with 5% FBS and the uptake of nanocarriers in the organoids was immediately visualized by confocal laser scanning microscope (Olympus Fluoview FV1000, Olympus Life Science Europa GmbH, Germany)

Since NAMPT, in the rate-limiting reaction of NAD + salvage pathway, consumes NAM, which acts as an endogenous inhibitor of SIRT1, to regenerate and boost NAD + pool, thus promoting SIRT1 activation

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