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Recombinant mouse HGF (carrier protein-free) was incubated with GSSSG (A) and LASSS (B) at molar ratios ranging from 1:0 to 1:4000 relative to HGF (corresponding to weight ratios of 1:0 to 1:57 and 1:0 to 1:21 with A and B, respectively) just prior to the optimized peroxynitrite-protocol at a molar ratio 1:2000 relative to HGF, pH 7.4 (Figure 1b,c)
dorsalis adults were dissected and fixed in 4% paraformaldehyde in PBS (pH 7.2) for 2 h at room temperature
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Official labeling states that bacteriostatic water contains water plus either 0.9 percent, equal to 9 mg/mL, or 1.1 percent, equal to 11 mg/mL, benzyl alcohol
