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Complex was formed by adding 10 mM MgCl 2 , 1 mM MnCl 2 , 50 mU/mL apyrase, 30 M tirzepatide, 100 M TCEP and 10 g/mL Nb35 to the cell lysate and incubated at RT for 1.5 h
) Put the extracting needle on the 2ml syringe I then pull out the plunger on the syringe , and inject air SLOWLY into the ampoule
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