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Neutrophils were pelleted at 250 g for 6 min, washed in RPMI 1640 with 2% FBS, 2 mM L-glutamine and 10 mM HEPES, and centrifuged at 250 g for 4 min
Vitamin B12, also known as cobalamin, is a water-soluble vitamin that plays a fundamental role in several critical physiological processes
The resulting Gly-His-Lys-Cu(II) complex shifts the peptide's behavior from a passive ligand to an active modulator of intracellular copper trafficking [2]
We observed a dose-dependent correlation between the bioluminescence signal and intracellular ATP levels (Extended Data Fig
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