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Membranes were then probed with horseradish peroxidase-conjugated secondary antibodies antirabbit IgG (Abcam, A16035, 1:5,000) or antimouse IgG (Abcam, A16011, 1:5,000) and developed using enhanced chemiluminescence substrate (Bio-Rad, 170-5060)
The copper-peptide complex produces blue specifically
Simultaneous determination of multiple forms of lactate dehydrogenase based on NAD and thio-NAD
: 50 = 1 100 = 2 150 = 3 , - 15 150300 ( 50 100 )
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