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Apply gentle pressure post-injection

Of note, hemolysis is a fundamental feature of SCD and resulting excess hemin exposure 32 while hypoxia has been shown to induce the expression of fetal hemoglobin 33,34 , and promote the transition from expansion to differentiation during stress erythropoiesis 35

Primary hippocampal neuron cultures are used to study Dihexa's proposed effects on dendritic spine density and synaptic protein expression, typically measuring morphological changes via fluorescence microscopy and quantifying synaptic marker expression (such as PSD-95 or synaptophysin) relative to untreated controls

At the end of the experiment, brains were collected for cresyl violet staining to confirm cannula placement