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Description

Amino Acids 2009

Reference: Kleinman HK & Sosne G (2012)

After five washing steps with PBS-Tween, the secondary antibody (Alexa Fluor 555 anti-mouse, Life Technologies, 1:200) was added in 2% BSA/PBS-Tween together with Hoechst 33342 (1 g/ml) for 2 h

A few suppliers sell pre-blended cag/tirz vials (most often at a 5 mg / 5 mg ratio or similar), but the dose math is more flexible with separate vials

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