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The eluted proteins were subjected to size-exclusion chromatography (SEC) with a Superose 6 Increase 10/300 GL column (Cytiva) in the final buffer containing 25 mM HEPES pH 7.5, 150 mM NaCl and 1 mM DTT (buffer A, for biochemical assays) or 5 mM DTT (buffer B, for cryo-EM)
Other meta-analyses of berberine for hyperlipidemia, hypertension, or diabetes also determined that most studies are of limited quality (2) (37)
Most injections produce no bleeding
At clinical doses, the peptide activates collagen synthesis, accelerates cell turnover, and modulates the inflammatory response across multiple tissue types
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