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For elution of the target protein, the interaction to the ligand is resolved by changing the buffer conditions, for example, the pH value, or by adding a specific competitor that supplants the target protein from the ligand resulting in dissociation of the target, while the ligand remains immobilized on the stationary phase

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Doses of CoQ10 used in clinical studies in the context of doxorubicin toxicity have ranged from 30 mg to approximately 200 mg daily (Conklin 2005)