The stirring continued for an additional 12 h at 50 C, after which the mixture was introduced to 10 mL of deionized water while being sonicated
*Correspondence: Oh Yoen Kim, [email protected]
Front Cell Dev Biol 8:602574 Han X, Ding C, Zhang G, Pan R, Liu Y, Huang N, Hou N, Han F, Xu W, Sun X (2020) Liraglutide ameliorates obesity-related nonalcoholic fatty liver disease by regulating Sestrin2-mediated Nrf2/HO-1 pathway
Apple embryonic axes (25 mg were homogenised in 50 mM K-phosphate buffer pH 7.5 containing 5 mM DTT, 5% (w/v) glycerol, 0.1% (w/v) triton X-100, 0,1% (v/v) protease inhibitor cocktail and 2% (w/v) PVPP on ice