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end repair
The DNA from both reactions is then amplified using Real-Time PCR
It is designed for the rapid
Highlights: Sold separately or as part of the Zymo-Spin ChIP Kit (D5209
PureRec Duplex-Specific Nuclease (DSN) WGBS Library Kit end repairPureRec Duplex Specific Nuclease (DSN) is an endonuclease that selectively digests double stranded DNA (dsDNA) and DNA in DNA RNA hybrids, exhibiting virtually no activity towards single stranded DNA (ssDNA) or RNA. It can discriminate between perfectly matched and non matched short DNA duplexes. To catalyze cleavage, DSN needs a minimum of 10 bp dsDNA or 15 bp perfectly matched DNA RNA duplex. This enzymatic function is reliant upon the presence of
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