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Reduced roGFP2 was obtained after a 20 min treatment with 10 mM DTT, which was subsequently removed on a ZebaTM Spin Desalting Column (Thermo Scientific)
doi: 10.3389/fnins.2025.1537898 Received 03 December 2024 Accepted 03 March 2025 Published 18 March 2025 Volume 19 - 2025 Edited by Mark M
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This activation process strips the charcoal of previously absorbed molecules and frees up bonding sites again
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