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Cells were washed three times with Krebs buffer (20 mM HEPES, 5 mM KH 2 PO 4 , 1 mM MgSO 4 , 1 mM CaCl 2 , 136 mM NaCl, 4.7 mM KCl, pH 7.4), then incubated with 500 g/ml 2-NBDG, a fluorescence-labeled deoxyglucose analog, for 20 min at room temperature

If you have one of these conditions, youll need to take extra precautions during injections and work closely with your healthcare provider to manage your care

Polyphenolic proanthocyanidin-B2 suppresses proliferation of liver cancer cells and hepatocellular carcinogenesis through directly binding and inhibiting AKT activity