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The sodium citrate-buffered saline was removed using an Amicon Ultra-4 ultrafiltration tube with a molecular weight cut-off of 10,000 Da (Millipore, Bedford, MA, USA) at 4500 g and 4 C for 45 min

Japan clinically labels "obesity" at BMI25, so the eligible pool is larger than the BMI30 headline suggests, but still far below USA levels

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Suggested amount: Suggested amount will vary depending on your specific needs but typically between 100-600 milligrams per day is often recommended for men and women