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25 The Val 23 and Ile 27 used in tirzepatide are likely to have little impact on either GLP-1 or GIP activity because they are the same kind of residue and no specific interaction in these sites has been detected
60-80% of ATP generated derives from FA oxidation, the remainder from Glucose and Lactate metabolism
The drug was added at a concentration of (5 g/ml), in order to provoke only a slight inhibition of growth (final OD of about 1.0)
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