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Using the Roche LightCycler 480 II system (Roche Diagnostics, Germany), the procedure was carried out according to the following thermal protocol: an amplification phase comprising 40 cycles of 95C for 15 s and 60C for 60 s, followed by a cooling phase at 40C for 30 s
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The molecular mechanism of chronic stress affecting the occurrence and development of breast cancer and potential drug therapy
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