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Sections were dehydrated, treated with Triton X 100 (0.1% v/v) for 30 min and blocked in 1% bovine serum albumin for the same time
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Therefore, the changes in the gut microbiota after a single injection of GLP-1RA seem to reflect the original effects of GLP-1RA more clearly than the changes in the gut microbiota after chronic administration
Additional studies in cynomolgus monkeys have confirmed the potent dose-dependent, weight-lowering effects of chronic GLP-1R/GcgR co-agonism [78], supporting the translational promise of this novel balanced agonism for obesity and type 2 diabetes
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