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Manual validation ensured accurate identification of all amino acid residues and post-translational modifications. Nuclear Magnetic Resonance (NMR) Spectroscopy To complement mass spectrometric data, ^1H and ^13C NMR spectroscopy were performed, providing insights into the conformational aspects and confirming the presence of specific functional groups, particularly in modified regions. Quality Control and Batch-to-Batch Comparability Assessment (New Section) Beyond single-batch characterization, peptide sequencing plays a vital role in establishing batch-to-batch consistency during late-stage development

The lack of anti-atherosclerotic and plaque-stabilizing effects of incretin-based medications might contribute to elevated cardiovascular risk, especially acutely following their discontinuation

Ketones are produced by the liver when the body runs low on carbohydrates, typically during fasting or when following a low-carb, or ketogenic, diet

73 It has been shown that the changes in gut microbiome and concentrations of the aforementioned metabolites is important in NAFLD pathogenesis and progression, but a specific microbiome composition in NAFLD has not yet been identified