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RNA extraction, cDNA conversion, and qPCR RNA from 21NT, BT474, IBR.3, and HMEC was extracted, and mRNA gene expression analysis was performed (primers listed in Table 1) using qPCR as described previously (Al-dulaimi et al 2024)

However, there are also cases when additional factors such as lack of sleep, poor diet, and deficiency of other vitamins like iron, folate, and others, contribute to the issue

In wound healing models, GHK attached to biotin and bound to collagen pads produced higher levels of protein antioxidants in wound tissue

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