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When the cells reached approximately 80% confluency, cells were harvested with trypsin and seeded at a density of 2.9 10 5 cells per well in 2.5 ml of culture medium into 6 well flat-bottomed plates (Costar, Corning, USA) and incubated for 24 hr
Once the vial is opened or punctured, any bacteria introduced can grow unchecked
As for its role in protein synthesis, tryptophan is a precursor of crucial metabolites, such as 5-HT, which plays an essential role in functional gastrointestinal diseases ( The gut microbes in the third pathway can metabolize tryptophan into several molecules, such as indole and other derivatives compounds, with some of them acting as ligands to bind the aryl hydrocarbon receptor (AhR) (Williams et al., 2014
Many physicians choose to treat B12 deficiency with B12 shots because injections avoid the gastrointestinal tract entirely
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