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If upregulation of citrate synthase occurred due to excess PPA in vivo and then PPA was removed in vitro , the high activity of citrate synthase in vitro would overproduce citrate and enhance the proximal portion of the TCAC, which preferentially produces NADH, the electron carrier metabolized by complex I
Understanding this distinction prevents a common mistake where researchers add extra water to an already-reconstituted vial, which introduces contamination risk and makes concentration tracking nearly impossible
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