Ultimately, the choice of protocol is up to the lead researcher
"The two biggest killers in our country is cancer and cardiovascular disease
Membranes were blocked with 5% (w/v) milk powder in PBS for 1 h and incubated for 16 h at 4 C with one of the following primary antibodies: rabbit anti-SLC31A1/CTR1 (Abcam, Cambridge, UK), mouse anti-GAPDH (Santa Cruz Biotechnology, Dallas, TX, USA), mouse anti-XPF (Thermo Fisher, Waltham, MA, USA), mouse anti-ERCC1 (Santa Cruz Biotechnology), mouse anti-Tubulin (Abcam), rabbit anti-NRF2 (Abcam)
KLOW Blend Peptide (80mg Vial) High-Purity Research Compound The KLOW blend peptide is a research-grade peptide formulation supplied as an 80mg vial with bacteriostatic water, prepared for controlled laboratory environments studying peptide signaling and cellular pathways