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Without sufficient interventions to manage this pandemic, the number of people with diabetes worldwide is expected to increase to 578 million people (10.2% of the population) by 2030 and 700 million (10.9%) by 2045 [1]

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To establish a cell model of fat overloading, palmitic (C16:0) and oleic (C18:1) acids (free fatty acids, FFAs) were added into the culture solution at a final concentration of 0.5 mM at a 1:2 ratio 22,23

This mirrors the natural pattern of GH secretion, which occurs in 4-6 pulses per daypredominantly during deep sleep