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All controlled studies referenced below were conducted in cell culture (3T3-L1 adipocytes, primary muscle stem cells) or rodent models (diet-induced obese mice, aged C57BL/6J mice)
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In 2014, the US Food and Drug Administration (FDA) acknowledged a GRAS (Generally Recognised as Safe) notification for AOD 9604 as a food ingredient meaning the FDA raised no questions about the notifier's own safety conclusion
However, if serum cobalamin concentration is within the normal range, treatment should be continued at least monthly (for parenteral supplementation) and the owner should be forewarned that clinical signs may recur sometime in the future
