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Each reaction mixture was prepared using 10 l QuantiFast SYBR Green PCR master mix, 4 l of cDNA template with 1 M of each primer in a total reaction volume of 20 l
That is an unpleasant property in an experiment and an intolerable one in a manufacturing process
Van Cauter E, Spiegel K, Tasali E, Leproult R (2008) Metabolic consequences of sleep and sleep loss
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