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At 18 h after transfection, medium was replaced and cells were cultured for an additional 30 h prior to incubations

Although its exact mechanism in the prevention of pathogenesis is not entirely understood, the therapeutic activity of neem is thought to result from the synergistic/additive effects of its various bioactive compounds such as azadirachtin, nimbolinin, nimbin, nimbidin, nimbidol, sodium nimbinate, gedunin, salannin, and quercetin (Luong et al., 2012