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Transfection, BSO treatment, and exposure of AQ-QI and DEAQ-QI were identical as described in section Transient Transfection of Human GSTP1 Gene and Cell Viability Assay. After exposure the medium was gently removed and the later steps were conducted according to manufacturers manual

In a study involving temporarily induced insulin resistance through treatment of non-esterified fatty acid (NEFA), metformin improved extrahepatic glucose utilization in patients with T2DM potentially by promoting microvascular perfusion [50, 51]

Taking the injection after a light meal rather than on an empty stomach may reduce nausea for some individuals, though this is based on anecdotal experience rather than clinical trial data

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