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Samples were eluted using a gradient of Waters AccQTag buffers A and B over 17 min at 0.7 mL/min flow rate and 51 C column temperature with UV detection at 260 nm
These bioregulators work by interacting with specific cellular receptors or transcription factors, effectively communicating with target cells to enhance their physiological function
The macromolecular receptor was processed using AutoDockTools 1.5.7 software, including the addition of hydrogen bonds, calculation of charges, setting of rigid structures, and designation as the receptor, and then exported in PDBQT format
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