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On an experimental day, cells were first glucose-starved in KRB (0 mM glucose) for 1 h (37 C, 5% CO 2 ), followed by glucose stimulation with KRB supplemented with 20 mM glucose in the presence or absence of drugs for 90 min (37 C, 5% CO 2 )
This method allows for direct absorption of the vitamin into the bloodstream, bypassing any potential absorption issues in the digestive system
Without available vitamin B12, you may experience severe and permanent neurological challenges, such as paralysis, numbness, and problems controlling your bodys movements
For larger doses, a 0.5mL or 1mL syringe works better
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