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For more on spotting degradation, check do peptides expire
At the end of four hours, you can soak the patch for a few minutes with warm water to make the removal easier
Use sterile, single-use equipment only
The protein sequences of the selected differentially expressed proteins were locally searched using the NCBI BLAST+ client software (ncbi-blast-2.2.28+-win32.exe) and InterProScan to find homologue sequences, after which gene ontology (GO) terms were mapped, and sequences were annotated using the software program Blast2GO
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