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There are also some cases where specific GSTs are not able to conduct peroxidase activity, such as theta class GSTs [134, 135]

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The column oven temperature was 50 C, the mobile phase consisted of acetonitrile-methanol-water (30:16:54 v/v, pH 45 adjusted by 0.01 N HCl), the flow rate was 1.2 ml/min and the eluate absorbance was monitored online at a wavelength of 230 nm