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Fukuda K, Asoh S, Ishikawa M, Yamamoto Y, Ohsawa I, Ohta S
26S proteasome activity assay Briefly, pulverized liver samples (20 mg aliquot) were homogenized in 26 S proteasome lysis buffer (50 mM Tris, 150 mM sodium chloride (NaCl), 1 mM ethylenediaminetetraacetic acid (EDTA), 5 mM magnesium chloride (MgCl 2) [pH 7.5]) with a hand-held Potter-Elvehjem homogenizer
Its activity is studied through copper transport and signaling interactions rather than one receptor
Research published in the journal Oxidative Medicine and Cellular Longevity identified over 4,000 genes affected by GHK treatment
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