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Material and methods Cell culture Human hepatocellular carcinoma (HepG2) (ATCC, HB-8065) were maintained in DMEM (4.5 g/l glucose, pyruvate) (Gibco), supplemented with 10% fetal bovine serum (PAA Laboratories) and penicillin/streptomycin (25 g/ml each, Gibco) at 37 C, 5% CO 2

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After reconstitution, store at 2-8C and use within 30 days

Co-eluting, high abundant interferences in TOFMS survey scans resulted in missed MS/MS acquisition of target peptides