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Interestingly, endogenous GLP-1 can only exist for less than 3 minutes in systematic circulation, while its effects can only be felt locally
For immunofluorescence, the primary antibodies used were monoclonal [8G9] to GLP-1, monoclonal SST antibody (#MAB2358), and monoclonal Human/Mouse Ghrelin Antibody (R&D systems #MAB8200), and the secondary antibodies were Alexa Fluor 488 AffiniPure Donkey Anti-Rat IgG and Cyanine Cy3 AffiniPure Donkey Anti-Mouse IgG from Jackson ImmunoResearch (Europe Ltd.) and were used at a final dilution of 1/300
Efficacy and safety of the new appetite suppressant, liraglutide: A meta-analysis of randomized controlled trials
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