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Cultures were pulse-labeled with 10 M BrdU (a nucleoside analog that competes with thymidine for incorporation into DNA) in DMEM-10% HS for the final 2-h period of 48-h in culture, followed by immunocytochemistry for detection of BrdU using G3G4 anti-BrdU mAb (1:100 dilution) and HRP-conjugated anti-mouse IgG Ab (1:500 dilution)

During this process, GSK3 is a downstream target of insulin-activated signaling and is an important enzyme involved in the control of glycogen metabolism [48]

Nihon Koshu Eisei Zasshi , 55 (5), 327-340

Besides, there is little research regarding the long-term impacts of glutathione injections for skin whitening