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NTS were purified by layering on 4 C Percoll gradient suspension containing Eagles minimum essential medium, penicillin-streptomycin (10,000 U per ml penicillin/10,000 U per ml streptomycin), and 1 M HEPES in 0.85% NaCl with cercariae suspension and centrifuged at 500 g for 15 min

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TL;DR: Bacteriostatic water remains safe for 28 days after first puncture if stored properly at 2C8C