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Samples (whole cells or isolated mitochondria) were freeze-thawed three times, and the lysates filtered through 10-kDa molecular mass cutoff devices (Amicon, Millipore) at 12,000 g for 15 min at 4 C
Silverman DH, Alavi A
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In exceptional circumstances, it may be considered for those with a BMI of 3034.9 kg/m with at least one weight-related comorbidity
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