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These AG 538 bioisosteres possessed similar but weaker biological properties to AG 538 but are more stable and blocked the formation of colonies of prostate and breast cancer cells in soft agar systems (Blum et al, 2003)
The primer sequences for the MS2 IC detection are 5-ATTGCTTACTTAAGGGACGAATTG-3 and 5-GCTACGGATGCTGGTTTGT-3 for forward and reverse, respectively, and the 5 end of the reverse primer was labeled with TAMRA dye
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