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Hu and Chen (2018) showed that MOTS-c increased the expression of alkaline phosphatase (ALP), a marker of early osteoblast maturation, bone gamma-carboxyglutamic-acid-containing proteins (BGLAP), and Runx2, a transcription factor important for osteoblast differentiation, using the CCK-8 assay compared to negative controls

Initial Loading Phase: Daily Injections for One Week The initial phase involves daily vitamin B12 injections for one week

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Trnwall ME, Virtamo J, Korhonen PA, Virtanen MJ, Taylor PR, Albanes D, Huttunen JK