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Wash hands thoroughly Clean the tops of both vials with alcohol swabs Draw your chosen solvent into a sterile syringe Inject the solvent slowly into the IGF-1 LR3 vial, directing the stream against the glass wall rather than onto the powder Let the vial sit undisturbed for several minutes, allowing the peptide to dissolve naturally Gently swirl to mix, never shake vigorously Confirm the solution is clear with no visible particles Label with date and concentration For a 1 mg vial, adding 1 mL of solvent creates a concentration of 1000 mcg/mL
Determination of cell death by PI staining Cells were trypsinized, washed with PBS and suspended in 300 l Dulbeccos phosphate buffered saline (DPBS) supplemented with 0.2% BSA containing 0.3 g/ml propidium iodine (PI, Sigma-Aldrich)
Lean proteins: Grass-fed whey protein, poultry, and fish contain the necessary amino acids
and was applied in many parts of the world (5760)
