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The ETP toxin, sirodesmin, is produced by the fungus Leptosphaeria maculans with biosynthesis encoded by a multigene cluster similar to that responsible for gliotoxin production in A
Lig4 is unstable in the absence of XRCC4 12
Gas chromatography-mass spectrometry (GC-MS) analysis The MCF derivatives were analysed in an Agilent GC7890 system coupled to a MSD5975 mass selective detector (EI) operating at 70 eV
To confirm that these proteins were susceptible to glutathionylation, we treated the recombinant protein with BioGEE in vitro and analysed the extent of BioGEE labelling by Western blot using streptavidin-HRP
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