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Yet, the quest for effective and safe weight loss solutions persists, and GLP-1 has continued to make waves since its FDA approval in 2021 as a highly effective weight loss treatment
Cell culture Rat pheochromocytoma cells (PC12) were obtained from the American Type Culture Collection (Manassas, VA, USA), and were cultured in Dulbeccos Modified Eagles medium supplemented with 6% fetal bovine serum (heat inactivated), 6% horse serum (heat inactivated), 1 mm glutamine, 100U ml 1 penicillin and 100 g ml 1 streptomycin at 37 C in a humidified 5% CO 2 incubator
Visual analog scale ratings confirmed that pramlintide-treated subjects reported greater fullness and reduced hunger compared to placebo
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