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After further washes in PBST, blots were imaged with the Odyssey infrared imaging system (LiCore Biosciences)

2 Department of Internal Medicine, Lahore General Hospital, Ameer-Ud-Din Medical College, Lahore, Punjab, Pakistan Correspondence: Hongwei Jiang, Department of Metabolism and Endocrinology, Endocrine and Metabolic Disease Center, The First Affiliated Hospital and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, Peoples Republic of China, Email [email protected] Abstract: Adolescent obesity is an escalating global health concern associated with increased risks of cardiovascular disease, type 2 diabetes mellitus, and long-term metabolic complications

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