doi:10.1016/j.urology.2020.07.087
Exogenous GSH has the potential to scavenge free radicals to increase the competence of oocyte development in vitro in bovine and porcine, particularly in oocytes lacking cumulus cells []
Bardos J, Fiorentino D, Longman RE, et al
After blocking in 5% skimmed milk powder for 2 hours at room temperature, the samples were incubated overnight at 4C with primary antibodies, including ERK1/2 (#9102), p-ERK1/2 (#9101), JNK1/2 (#9252), p-JNK (#9251), p38 MAPK (#9212), and p-p38 MAPK (#9211), which were obtained from Cell Signaling Technology (Beverly, MA, USA), as well as VEGF-a (sc-7269) and Tubulin (sc-8035), which were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA)