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% Inhibition = [(blank ODSample OD)/blank OD] 100 Determination of reduced glutathione (GSH) level GSH level was measured in serum samples in both refractory epilepsy and healthy control groups

Trp53 fl/fl (AKP) mice 72 h post-tamoxifen intraperitoneal (i.p.) injection and cultured in 3D as previously reported (Extended Data Fig

Maintenance dose optimization Once reaching the initial target dose, assessment determines whether further increases benefit the protocol

Studies tracking patients over 2 years show that those who stay on their maintenance dose sustain weight loss, while those who stop gain weight back over 3 to 6 months