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Cellular viability Optimization of Sp concentration and its cytotoxicity parameters were established by treating of 1.5 10 5 HeLa or 1.2 10 5 SHSY-5Y cells with serial concentrations of the Sp (100 M1 mM) for 15 min or conducting a time-course (5 min48 h) using 250 M Sp, followed by MTT assay as per standard protocol (Thermo Fisher Scientific)
Baseline Assessment IGF-1 serum levels Fasting glucose and HbA1c Comprehensive Metabolic Panel Cancer screening when appropriate Complete Blood Count Follow-Up Monitoring Monthly fasting glucose IGF-1 levels at 46 weeks Clinical assessment during follow-up visits Metabolic monitoring every three months Clinical Evidence Summary Several published studies support the potential applications of IGF-1 LR3 in muscle development, tissue repair, and metabolic optimization
The overall reduction in insulin secretion is also shown by reduced levels of C-peptide in the same studies
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