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One of the principal concerns surrounding engineered probiotics is the potential for horizontal gene transfer, including the dissemination of GLP-1 expression cassettes or their regulatory elements into wild-type microbial populations
Notably, however, the unstable product flavan-3,3,4-triol ( 4 ) is converted mainly into the by-products epidihydroquercetin ( 9 ), dihydroquercetin ( 10 ) and quercetin ( 11) 13 , while the transformation into the coloured anthocyanidin has only been observed as a minor side reaction in vitro 11,12,13 or in vivo by engineered microorganisms 6,16
Notably, physical activity and arterial blood oxygen saturation are parameters easily obtainable without simulating high-altitude environments
This activation occurs not only as a response to stress but also under conditions of impaired autophagy via an alternative SQSTM1 p62-mediated pathway, the levels of which are also elevated (Marques et al., 2022)
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