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Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]
It plays a critical role in detoxifying the body, neutralizing toxins, and maintaining cellular integrity
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doi: 10.1038/nri2448 24 ZhouJTangZGaoSLiCFengYZhouX
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